id = "sratoolkit" name = "SRA Toolkit" homepage = "https://github.com/ncbi/sra-tools" description = "A toolkit for using data in the INSDC Sequence Read Archives." executables = ["prefetch", "fasterq-dump", "vdb-config"] [dotfiles] paths = ["~/.ncbi/user-settings.mkfg"] secret = [] # NCBI's official SRA-tools documentation identifies ~/.ncbi/user-settings.mkfg # as the preconfigured user settings file used by the toolkit. # https://github.com/ncbi/sra-tools/wiki/SRA-tools-docker [commands] format = 1 label = "Download SRA accessions (prefetch)" description = "Download one or more SRA run accessions with prefetch, resumable on failure. Only prefetch is covered; fasterq-dump and vdb-config have no form." quality = "ai-draft" parser = { end_of_options = "--", interleave = "permute", long_equals = true, short_attached = true, short_bundles = false } [[commands.source]] id = "wiki" kind = "manual" url = "https://github.com/ncbi/sra-tools/wiki/08.-prefetch-and-fasterq-dump" revision = "2026-10-27" evidence = """ $prefetch SRR000001 -O /path/to/be/used Make sure the last directory of /path/to/be/used is the accession itself. $prefetch SRR1951777 --max-size 420000000000 kilobytes (default): --max-size 10 == --max-size 10k : 10 kilobytes, megabytes: --max-size 10m : 10 megabytes, gigabytes: --max-size 10g : 10 gigabytes, terabytes: --max-size 10t : 10 terabytes, unlimited: --max-size u. If prefetch fails - run the same prefetch command again and the download will resume. """ [[commands.field]] id = "accessions" label = "Accessions" description = "SRA run accessions such as SRR000001." type = "string" position = 0 min = 1 multiple = true sources = ["wiki"] [[commands.field]] id = "output_dir" label = "Output directory" description = "The last directory should be named after the accession." type = "path" option = ["-O"] path = { kind = "directory", role = "output" } sources = ["wiki"] [[commands.field]] id = "max_size" label = "Maximum download size" description = "Default limit is 20G. Suffixes k, m, g, t; u means unlimited." type = "string" option = ["--max-size"] suggestions = [{ value = "u", label = "Unlimited" }, { value = "100g", label = "100 GB" }] sources = ["wiki"] [[commands.task]] id = "download" label = "Download accessions" fields = ["accessions", "output_dir"] required = ["accessions"] [[commands.task]] id = "large" label = "Download a large accession" fields = ["accessions", "max_size", "output_dir"] required = ["accessions", "max_size"] set = { max_size = ["u"] } [[commands.group]] id = "basic" label = "Basic" fields = ["accessions", "output_dir"] [[commands.group]] id = "advanced" label = "Advanced" fields = ["max_size"]