BLAST+

blast

Basic Local Alignment Search Tool

Runs as blastn, blastp, blastx, tblastn, tblastx, makeblastdb|macOS, Linux, Windows|blast.ncbi.nlm.nih.gov

Open form in Otty otty.toml

Command form

ai-draft · 1 task · 5 fields

Search a nucleotide query file against a BLAST database with blastn. Only the flags shown in NCBI's BLAST+ documentation examples are offered; the other BLAST+ programs and tuning options are left out.

Opens the form; the finished command lands at your prompt, unrun

Run in Otty

otty gui blast

BLAST+ blastnExample values, fixed in this page — nothing here reaches a terminal

Basic

Query file*

file

Database*

string

Output file

file

Threads

integer

Task

string

TasksEach one presets the form for one job

Search a database

querydboutnum_threadstask

ParametersOnly these flags are editable in the form

Query file

path

-query

Database

BLAST database name, for example nt.
string

-db

Output file

path

-out

Threads

integer

-num_threads

Task

Without -task, blastn runs the MegaBLAST algorithm.
string

-task

SourcesEvery flag above is checked against these

docs

blastn -query /blast/queries/query1.fa -db nt -num_threads 16 \ -out /blast/results/blastn.query1.denovo16s.out Please note that running the `blastn` binary without specifying its `-task` parameter invokes the MegaBLAST algorithm.

help2026-10-27

raw.githubusercontent.com

Before it fills your prompt, Otty checks the installed blastn against its version. A form that does not match your version is not offered.